Flow Cytometry - Facts

Flow cytometry is a powerful, versatile technology for multiparametric, high-throughput analysis of single cells in suspension. A large variety of flow cytometry-based assays have been developed to assess and quantify key aspects of cell function at single cell level.
Below are some common applications of flow cytometry, which can be used during genetic and chemical screenings and which can also be employed as readouts for the relevant screen endpoints:

• Characterization of heterogeneous cell populations
• Purification of specific cell populations (cell sorting)
• Measurement of cell viability, proliferation and cell cycle progression
• Multiparametric analysis of cell surface/intracellular markers
• Analysis of fluorescent reporters
• Assessment of different aspects of cell physiology (reactive oxygen species, mitochondrial fitness, phagocytosis, inflammasome activation, induction of apoptosis,…)
• Measurement of secreted inflammatory mediators with bead-based multiplex assays

Visit the FCCF webpage for a collection of useful links on flow cytometry and related cellular assays.